PINK1 protein in normal human brain and Parkinson's disease
Identifieur interne : 001148 ( Main/Exploration ); précédent : 001147; suivant : 001149PINK1 protein in normal human brain and Parkinson's disease
Auteurs : S. Gandhi ; M. M. K. Muqit ; L. Stanyer ; D. G. Healy ; P. M. Abou-Sleiman ; I. Hargreaves ; S. Heales ; M. Ganguly ; L. Parsons ; Andrew Lees (neurologue) [Royaume-Uni] ; D. S. Latchman [Royaume-Uni] ; J. L. Holton ; N. W. Wood ; T. ReveszSource :
- Brain [ 0006-8950 ] ; 2006-05-15.
Abstract
Parkinson's disease is a common incurable neurodegenerative disease whose molecular aetiology remains unclear. The identification of Mendelian genes causing rare familial forms of Parkinson's disease has revealed novel proteins and pathways that are likely to be relevant in the pathogenesis of sporadic Parkinson's disease. Recently, mutations in a novel gene, PINK1, encoding a 581 amino acid protein with both mitochondrial targeting and serine/threonine kinase domains, were identified as a cause of autosomal recessive parkinsonism. This provided important evidence for the role of the mitochondrial dysfunction and kinase pathways in neurodegeneration. In this study, we report the first characterization of the PINK1 protein in normal human and sporadic Parkinson's brains, in addition to Parkinson's cases with heterozygous PINK1 mutations. The possible role of the PINK1 protein was also assessed in a number of neurodegenerative diseases characterized by proteinaceous inclusions. For these studies, rabbit polyclonal antibodies were raised against two peptide sequences within the N-terminal hydrophilic loops of PINK1 protein. Using immunohistochemistry and western blotting we were able to demonstrate that PINK1 is a ubiquitous protein expressed throughout the human brain and it is found in all cell types showing a punctate cytoplasmic staining pattern consistent with mitochondrial localization. Fractionation studies of human and rat brain confirm that PINK1 is localized to the mitochondrial membranes. In addition, we show that PINK1 is detected in a proportion of Lewy bodies in cases of sporadic Parkinson's disease and Parkinson's disease associated with heterozygous mutations in the PINK1 gene, which are clinically and pathologically indistinguishable from the sporadic cases. PINK1 was absent in cortical Lewy bodies, in neurofibrillary tangles in Alzheimer's disease, progressive supranuclear palsy and corticobasal degeneration, and in the glial and neuronal -synuclein positive inclusions in multiple system atrophy. These studies provide for the first time in vivo morphological and biochemical evidence to support a mitochondrial localization of PINK1 and underpin the significance of mitochondrial dysfunction in the pathogenesis of nigral cell degeneration in Parkinson's disease.
Url:
DOI: 10.1093/brain/awl114
Affiliations:
- Royaume-Uni
- Angleterre, Grand Londres
- Londres
- National Hospital for Neurology and Neurosurgery, Université de Londres
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The identification of Mendelian genes causing rare familial forms of Parkinson's disease has revealed novel proteins and pathways that are likely to be relevant in the pathogenesis of sporadic Parkinson's disease. Recently, mutations in a novel gene, PINK1, encoding a 581 amino acid protein with both mitochondrial targeting and serine/threonine kinase domains, were identified as a cause of autosomal recessive parkinsonism. This provided important evidence for the role of the mitochondrial dysfunction and kinase pathways in neurodegeneration. In this study, we report the first characterization of the PINK1 protein in normal human and sporadic Parkinson's brains, in addition to Parkinson's cases with heterozygous PINK1 mutations. The possible role of the PINK1 protein was also assessed in a number of neurodegenerative diseases characterized by proteinaceous inclusions. For these studies, rabbit polyclonal antibodies were raised against two peptide sequences within the N-terminal hydrophilic loops of PINK1 protein. Using immunohistochemistry and western blotting we were able to demonstrate that PINK1 is a ubiquitous protein expressed throughout the human brain and it is found in all cell types showing a punctate cytoplasmic staining pattern consistent with mitochondrial localization. Fractionation studies of human and rat brain confirm that PINK1 is localized to the mitochondrial membranes. In addition, we show that PINK1 is detected in a proportion of Lewy bodies in cases of sporadic Parkinson's disease and Parkinson's disease associated with heterozygous mutations in the PINK1 gene, which are clinically and pathologically indistinguishable from the sporadic cases. PINK1 was absent in cortical Lewy bodies, in neurofibrillary tangles in Alzheimer's disease, progressive supranuclear palsy and corticobasal degeneration, and in the glial and neuronal -synuclein positive inclusions in multiple system atrophy. These studies provide for the first time in vivo morphological and biochemical evidence to support a mitochondrial localization of PINK1 and underpin the significance of mitochondrial dysfunction in the pathogenesis of nigral cell degeneration in Parkinson's disease.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement><orgName><li>National Hospital for Neurology and Neurosurgery</li><li>Université de Londres</li></orgName></list><tree><noCountry><name sortKey="Abou Sleiman, P M" sort="Abou Sleiman, P M" uniqKey="Abou Sleiman P" first="P. M." last="Abou-Sleiman">P. M. Abou-Sleiman</name><name sortKey="Gandhi, S" sort="Gandhi, S" uniqKey="Gandhi S" first="S." last="Gandhi">S. Gandhi</name><name sortKey="Ganguly, M" sort="Ganguly, M" uniqKey="Ganguly M" first="M." last="Ganguly">M. Ganguly</name><name sortKey="Hargreaves, I" sort="Hargreaves, I" uniqKey="Hargreaves I" first="I." last="Hargreaves">I. Hargreaves</name><name sortKey="Heales, S" sort="Heales, S" uniqKey="Heales S" first="S." last="Heales">S. Heales</name><name sortKey="Healy, D G" sort="Healy, D G" uniqKey="Healy D" first="D. G." last="Healy">D. G. Healy</name><name sortKey="Holton, J L" sort="Holton, J L" uniqKey="Holton J" first="J. L." last="Holton">J. L. Holton</name><name sortKey="Muqit, M M K" sort="Muqit, M M K" uniqKey="Muqit M" first="M. M. K." last="Muqit">M. M. K. Muqit</name><name sortKey="Parsons, L" sort="Parsons, L" uniqKey="Parsons L" first="L." last="Parsons">L. Parsons</name><name sortKey="Revesz, T" sort="Revesz, T" uniqKey="Revesz T" first="T." last="Revesz">T. Revesz</name><name sortKey="Stanyer, L" sort="Stanyer, L" uniqKey="Stanyer L" first="L." last="Stanyer">L. Stanyer</name><name sortKey="Wood, N W" sort="Wood, N W" uniqKey="Wood N" first="N. W." last="Wood">N. W. Wood</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Lees, A J" sort="Lees, A J" uniqKey="Lees A" first="A. J." last="Lees">Andrew Lees (neurologue)</name></region><name sortKey="Latchman, D S" sort="Latchman, D S" uniqKey="Latchman D" first="D. S." last="Latchman">D. S. Latchman</name><name sortKey="Lees, A J" sort="Lees, A J" uniqKey="Lees A" first="A. J." last="Lees">Andrew Lees (neurologue)</name><name sortKey="Lees, A J" sort="Lees, A J" uniqKey="Lees A" first="A. J." last="Lees">Andrew Lees (neurologue)</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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